FastQCFastQC Report
Wed 25 May 2016
SRR1781113_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781113_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences289501
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT4250014.680432882787969No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG76432.640059965250552No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC31401.0846249235753935No Hit
ATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTT16160.5582018714961261No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC16030.5537113861437438No Hit
ATTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT14970.5170966594243198No Hit
CTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC9670.3340230258271992No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAATAAATCTCGTATGCCGT9450.32642374292316784No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4760.16442084828722528No Hit
CTTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG4070.14058673372458125No Hit
CTTATACACATCTCCGAGCCAACGAGACCCAAATAAATCTCGTATGCCGT3960.13678709227256555No Hit
TTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC3710.1281515435179844No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC3480.12020683866376973No Hit
CTTTACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC3470.11986141671358648No Hit
TATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCT3230.11157128990918856No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAAAAAATCTCGTATGCCGT3080.10638996065643988No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATAATCTCGTATGCCGTC2940.10155405335387443No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCAAC252.8364138E-669.9967248
TAAGACG207.920457E-569.9967243
AAACTCG1000.066.4968836
AGGGGGG55350.062.97807770
AACTCGT900.058.330637
ATCTCGA1100.057.27004237
CGTATGA1500.055.9973841
ACGACAA252.3885933E-455.99737550
TCTCGAA1200.055.4140738
AGCCGTC700.054.9974245
CCGTGCG200.00694335452.5066079
GACGTAT200.006948099552.4975446
CCGTCAA200.006948099552.4975447
TATGACG1200.052.4975443
AATATCG1600.050.31014336
ATGACGT1400.049.99765844
CGTCTTC73950.049.8827248
GCCGTCT74500.049.70236646
TGCCGTC75050.049.6645645
CCGTCTT73800.049.65214547
Produced by FastQC (version 0.11.5)