FastQCFastQC Report
Wed 25 May 2016
SRR1781112_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781112_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122694
Sequences flagged as poor quality0
Sequence length76
%GC80

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7000357.05494971229237No Hit
CTTATACACATCTGACGCTGCCGACGACAGGAACAGTGTAGATCTCGGTG2610.21272433859846449No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTGG150.002220309369.998783
CTCCGAG150.002220309369.9987864
GCGCGTC252.826173E-669.998786
TGTGCGC150.002220309369.998783
CAGGGCT150.002220309369.998784
GGCGCGT150.002220309369.998785
CGCGTCT207.90114E-569.998787
GCGCTGC150.002220309369.9987815
GTGTGCG252.3780027E-456.021862
CATGGGC200.00693779352.4990866
CGAGTGG200.00693779352.4990825
TGAACTA200.00693779352.499084
GCAAAGA200.00693779352.4990828
CGTCTGG407.4300624E-752.499089
TGCGCGT200.00693779352.499085
CTAAAAG200.00693779352.4990814
GCGGGAT200.00693779352.499086
CTTTGCC200.00693779352.499083
CCAACAG200.00693779352.4990824
CCTGCAT200.00693779352.4990817
Produced by FastQC (version 0.11.5)