FastQCFastQC Report
Wed 25 May 2016
SRR1781112_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781112_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122694
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT4108433.484929988426494No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG75846.181231356056531No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC28962.360343619084878No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC14941.2176634554256933No Hit
ATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCTT11970.9755978287446818TruSeq Adapter, Index 7 (95% over 21bp)
ATTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT11370.9266956819404373No Hit
CTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC7960.6487684809363131No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTAGTATGCCGT4800.3912171744339577No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4680.3814367450731087No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCACGTATGCCGT4540.37002624415211827No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGACGT4160.33905488450943No Hit
CTTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG3510.28607755880483154No Hit
TTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC3510.28607755880483154No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTAAGCCGT3340.2722219505436289No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAGGGTCATCTCGTATGCCGT2870.23391526888030387No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC2750.22413483951945493No Hit
TATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCT2730.22250476795931343No Hit
CTTATACACATCTCCGAGCCAACGAGACCTTGGGTCATCTCGTATGCCGT2540.20701908813796926No Hit
CTTTACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC2450.19968376611733252No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGGGTCATCTCGTATGCCGT2250.18338305051591763No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCAGT2200.1793078716155639No Hit
CCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG2050.16708233491450275No Hit
CTTATACACATCTCCGAGACCACGAGACCTTGGGTCATCTCGTATGCCGT1830.14915154775294637No Hit
CTTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG1800.14670644041273412No Hit
TCTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT1640.1336658679316022No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATATCGTATGCCGT1590.12959068903124846No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTAGTATGCCG1460.11899522389032878No Hit
CTTATACACATCTCAGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT1390.11328997342983356No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGA1340.10921479452947985No Hit
CTTATACACATCTCCGAGCCCACGAGACCATGGGTCATCTCGTATGCCGT1290.10513961562912612No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGAGT150.002216976170.025291
ACATTGA150.002216976170.025296
ATAGATT150.002216976170.025294
TTAGTTA150.002216976170.025298
TAGATTG150.002216976170.025295
GATCCAT207.90228E-569.9967431
TGACGAA207.90228E-569.9967445
ATACGAG252.8266622E-669.9967412
TCCATCG150.002220566269.99673533
TATGGCG150.002220566269.99673545
CGTTGGT150.002220566269.99673539
TGGCGTC150.002220566269.99673547
TCCCTAG150.002220566269.99673520
TTCCGTT150.002220566269.99673536
TGTGATC150.002220566269.99673528
TATTGGA150.002220566269.99673551
CGAATTA150.002220566269.99673548
GTTCCGT150.002220566269.99673535
ATTGGAT150.002220566269.99673552
AACTCGT301.016997E-769.99673537
Produced by FastQC (version 0.11.5)