FastQCFastQC Report
Wed 25 May 2016
SRR1781111_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781111_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334001
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7738223.16819410720327No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGTGC150.002219617170.0311
TCTCGGG207.920651E-569.9995543
TCGTTAA252.3886492E-455.9996423
CGTTAAT252.3886492E-455.9996424
TGCGCGT452.6959242E-854.4440965
CGCGTCT452.6959242E-854.4440967
TGCGGGC200.00694791952.4996619
TATGCGC200.00694791952.499663
TGTCTGC200.00694791952.4996665
TTACGTC200.00694791952.4996633
TAAGATC200.00694791952.4996634
GCCGTAT1350.049.2589454
GTGCGCG506.184018E-848.9996874
CGTATCA1350.046.66636756
CGTGCGA305.873568E-446.66636710
GCGCGTC604.9221853E-946.6663676
GTATCAT1350.046.66636757
CGGTGGT1750.045.99970246
AGATCTC1550.045.16100340
TCGCCGT1400.044.9997152
Produced by FastQC (version 0.11.5)