FastQCFastQC Report
Wed 25 May 2016
SRR1781111_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781111_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334001
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT4805914.38887907521235No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG87912.632028047820216No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC33801.011973018044856No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC17620.5275433307085906No Hit
ATTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT17560.5257469289014105No Hit
ATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCTT15080.45149565420462817RNA PCR Primer, Index 19 (95% over 23bp)
CTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC10070.3014961033050799No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5490.16437076535698994No Hit
CTTATACACATCTCCGAGCCAACGAGACTTATGAAAATCTCGTATGCCGT4480.13413133493612295No Hit
TTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC4440.13293373373133613No Hit
CTTTACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC4240.12694572770740206RNA PCR Primer, Index 19 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG3890.11646671716551747No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC3520.10538890602123946No Hit
TATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCT3420.10239490300927243RNA PCR Primer, Index 19 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATATGAAAATCTCGTATGCCGT3410.10209550270807571No Hit
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCAGT3410.10209550270807571No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGACT150.002224480769.9923746
CGTCATA150.002224480769.9923748
AGGGGGG60500.062.93528470
CGACTTC750.060.6600548
GCCGTAA352.971774E-759.99345446
GACCACG1650.059.38746318
ATATCGT601.8189894E-1258.32697337
CGAGAAC308.372546E-658.32697315
TGCCGTA1500.058.3269745
ATATCCG1050.056.67745210
CATCTAA252.3863268E-456.0106669
AACGTAT252.3898609E-455.99389339
CGTCTAC1000.055.99389348
TCTACAG252.3898609E-455.99389350
GCCCTTC252.3898609E-455.99389342
ATGACGA252.3898609E-455.99389344
CGTAATC252.3898609E-455.99389348
AATAACG252.3898609E-455.99389336
AGCCGTC750.055.9938945
GCCGACT750.055.9938946
Produced by FastQC (version 0.11.5)