FastQCFastQC Report
Wed 25 May 2016
SRR1781110_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781110_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246985
Sequences flagged as poor quality0
Sequence length76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8444034.188311031034274No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGT207.916883E-569.9989855
TGACCAG150.002222958369.99898516
CGCGTCT150.002222958369.9989857
GCCGAGT252.3875208E-455.9991958
GAGGCAC252.3875208E-455.999197
GTTGTGC200.0069237852.5417941
GGTGTCG200.00694600552.4992441
CCTAGGT200.00694600552.499243
GTCTCGG200.00694600552.4992442
GACCAGG200.00694600552.4992417
ACCAGGG200.00694600552.4992418
AAACAGG352.0828502E-549.9992838
GTAAACA352.0828502E-549.9992836
GCCGTAT506.175651E-848.99929454
CGAGTTC305.87081E-446.66599360
GTGGCGC451.6805479E-646.66599312
CGGGGAG305.87081E-446.66599361
CCGAGTT305.87081E-446.66599359
GTGCGCG305.87081E-446.6659934
TGCCGAG305.87081E-446.66599357
Produced by FastQC (version 0.11.5)