FastQCFastQC Report
Wed 25 May 2016
SRR1781110_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781110_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246985
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT5804223.500212563516005No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG59522.409862947142539No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC29321.1871166265157802No Hit
ATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTT12820.5190598619349354Illumina PCR Primer Index 3 (95% over 21bp)
CTAATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT11680.4729032127457133No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC11210.45387371702734985No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT10040.40650241917525354No Hit
CTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTC8750.35427252667166026No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTC4210.17045569569002167No Hit
CATATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT3890.15749944328602952No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG3830.155070145960281No Hit
TTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTC3070.12429904650079965No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG3010.12186974917505111No Hit
TATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCT2770.11215255987205701No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTAATGAATCTCGTATGCCGT2470.10000607324331437No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAC150.00222331569.99615545
TGCTTTA207.9184705E-569.99615555
GACGTCA150.00222331569.99615546
GACGTAT150.00222331569.99615546
TCTTAAG150.00222331569.99615550
CGTCTAC650.069.99615548
TGACGTA207.9184705E-569.99615545
CCAGATT150.00222331569.99615518
CCGTCAA207.9184705E-569.99615547
ATATCGA150.00222331569.99615537
ACGTATT150.00222331569.99615547
GAACACG150.00222331569.99615518
CGTCAAC207.9184705E-569.99615548
TCGAAAG353.6998244E-969.99615540
CCACCAG150.00222331569.99615520
ATAACCG252.8353134E-669.9961510
AGGGGGG70350.065.8172770
CGAATGC800.065.6213941
CAACTCG1100.063.63286636
ATGCAAC950.062.62813633
Produced by FastQC (version 0.11.5)