FastQCFastQC Report
Wed 25 May 2016
SRR1781109_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781109_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100214
Sequences flagged as poor quality0
Sequence length76
%GC78

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5677556.65376095156366No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTGC150.002215639570.026461
TGCGGGC150.002215639570.0264619
CGGCGAG207.8981444E-569.99151622
GCGCGTG150.002220032769.9915166
CCGGCGA207.8981444E-569.99151621
CGCGTGT150.002220032769.9915167
TGCCGGC308.305977E-658.3553819
TGCGCGT509.677024E-1055.993215
GCGTGTG252.3819086E-455.993218
ACCTAGA200.006923259252.5198441
GGGACTT200.00693694852.49363763
AAGGGTG200.00693694852.4936375
GTGCGCG407.421568E-752.4936374
CTGCCGG352.069093E-550.018918
GCGCGTC352.0752179E-549.993946
CGTGTGG305.8571034E-446.661019
CGCGTCT451.6717149E-646.6610077
TCTGGCG659.8789315E-943.07170511
GGCGCTG503.4401037E-642.01587714
GCGTCTG503.4519471E-641.9949078
Produced by FastQC (version 0.11.5)