FastQCFastQC Report
Wed 25 May 2016
SRR1781109_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781109_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100214
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT3927039.18614165685433No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG40614.052328018041392No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC18071.8031412776657954No Hit
ATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCTT7200.7184624902708204RNA PCR Primer, Index 34 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC6930.6915201468856647No Hit
CTAATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT6410.6396311892549943No Hit
CTTAAACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT5660.5647913465184505No Hit
CTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC4880.48695791007244493No Hit
CTTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG2650.26443411100245473No Hit
CATATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT2500.249466142455146No Hit
CTTTACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC2280.22751312191909315RNA PCR Primer, Index 34 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTAGTATGCCGT2060.20556010138304026No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGATCTCGTATGCCGTC1980.19757718482447564No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATATCGTATGCCGT1910.19059213283573154No Hit
CTTAATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG1860.1856028099866286No Hit
TTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC1820.1816113517073463No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCAAGGGATCTCGTATGCCGT1760.17562416428842278No Hit
TATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCT1710.17063484143931984RNA PCR Primer, Index 34 (95% over 22bp)
TCTTAATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC1650.16464765402039636No Hit
CTTATACACATCTCCGAGCCCACGAGACATCATGGGATCTCGTATGCCGT1560.1556668728920111No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGACGT1460.14568822719380525No Hit
CTTATACACATCTCCGAGCCAACGAGACCTCATGGGATCTCGTATGCCGT1450.14469036262398466No Hit
CCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG1270.12672880036721418No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCAGT1070.10677150897080248No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTAAGCCGT1050.1047757798311613No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGTGG150.002215075370.030965
GTCATCA301.0151598E-769.9960149
GCAAGAA150.002219467769.9960156
GCTGGTT150.002219467769.9960127
GTCTACT207.895635E-569.9960149
TGACGTA207.895635E-569.9960145
CTGGTTG150.002219467769.9960128
GCTCTCG150.002219467769.9960136
ATAACCG150.002219467769.9960110
AACTCGT150.002219467769.9960137
ACGTAAG207.895635E-569.9960140
TGACTTG150.002219467769.9960146
CTTAAAC1750.066.029181
ATGACGT850.065.878644
AGGGGGG47250.065.4036770
ATGGGAA600.064.1630132
TCACCGA550.063.63273611
TCTTTAT1000.063.027861
TATGACG950.062.62800643
TGGGAAC453.8016879E-1062.2186733
Produced by FastQC (version 0.11.5)