FastQCFastQC Report
Wed 25 May 2016
SRR1781108_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781108_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113456
Sequences flagged as poor quality0
Sequence length76
%GC89

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8232372.55940628966296No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1310.11546326329149627No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1180.10400507685798902No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGCG207.89815E-569.99956511
TTGTTAG150.002219780869.9995651
CTGGCGC252.8246177E-669.9995612
GCGCTGC301.0160329E-769.9995615
AGTAAAT150.002219780869.9995613
TGTGCGC150.002219780869.999563
GTAAATT150.002219780869.9995614
TGTCGGT150.002219780869.9995643
TGCCGGC308.332023E-658.33296619
CTGCCGG308.332023E-658.33296618
TCCTACT200.006936150652.4996722
TTTTCCT200.006936150652.49967244
GTATCAT200.006936150652.49967257
CCGTATC200.006936150652.49967255
CGAACAG200.006936150652.49967230
CGTATCA200.006936150652.49967256
GCCGTAT200.006936150652.49967254
CCGGCGA200.006936150652.49967221
GGTGGTC352.0755633E-549.99968747
GTGGTCG352.0755633E-549.99968748
Produced by FastQC (version 0.11.5)