FastQCFastQC Report
Wed 25 May 2016
SRR1781108_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781108_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113456
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT5628649.61042166126075No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG57125.034550839091807No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC25702.265195318008743No Hit
CTAATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT11110.9792342405866591No Hit
ATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTT10920.9624876604146101TruSeq Adapter, Index 10 (95% over 21bp)
CTTAAACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT10090.8893315470314482No Hit
TCTTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC9380.8267522211253702No Hit
CTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC7090.6249118601043576No Hit
CTTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG4090.36049217317726695No Hit
CATATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT3490.3076082357918488No Hit
CTTTACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC3110.2741150754477507No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCTATCTCGTATGCCGTC3110.2741150754477507No Hit
CTTATACACATCTCCGAGCCCACGAGACACAGGCTAATCTCGTATGCCGT3020.26618248483993795No Hit
CTTATACACATCTCCGAGCCAACGAGACACCGGCTAATCTCGTATGCCGT2620.23092652658299254No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATATCGTATGCCGT2430.21417994641094346No Hit
TCTTAATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC2210.19478916936962345No Hit
TATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCT2190.1930263714567762No Hit
TTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC2180.19214497250035256No Hit
CTTAATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG2080.1833309829361162No Hit
CTAAAACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT1990.17539839232830348No Hit
CCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG1810.15953321111267804No Hit
CTTATACACATCTCCGAGACCACGAGACACCGGCTAATCTCGTATGCCGT1570.1383796361585108No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCAGT1560.13749823720208715No Hit
CTTATACACATCTCCGAGCCCACGAGACCCCGGCTAATCTCGTATGCCGT1550.13661683824566354No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1470.12956564659427444No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGACGT1450.12780284868142716No Hit
CTTATACACATCTCAGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT1360.11987025807361443No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCAAATCTCGTATGCCGT1290.11370046537864899No Hit
CTTATACACATCTCCGAGCACACGAGACACCGGCTAATCTCGTATGCCGT1260.11105626850937808No Hit
TCTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT1210.10664927372725991No Hit
TCTAATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG1150.10136087998871808No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGGCTAATCTCGTATGCCGT1140.10047948103229447No Hit
CTTATACACATCTCCGAGCCCACGAGCCACCGGCTAATCTCGTATGCCGT1140.10047948103229447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCAC252.8245104E-670.0000117
ACACGAA150.002219724770.0000138
AACCGAG301.01597834E-770.0000112
ACAGAAT150.002219724770.0000153
AGCCAAA150.002219724770.0000117
GATACAC150.002219724770.0000124
CCACGAT150.002219724770.0000120
CACCGAT150.002219724770.0000112
CTGACGT150.002219724770.0000144
GACACCT252.8245104E-670.0000126
AACTCGA150.002219724770.0000137
GACATCT252.8245104E-670.0000149
GCAAACG301.01597834E-770.0000118
GACGTAT150.002219724770.0000146
AAGCTTG252.8245104E-670.0000154
AAGCCGA301.01597834E-770.0000144
TGACGAA252.8245104E-670.0000145
TCCCACG207.897904E-570.018
AAATCGT353.6743586E-970.037
GGCAAAA207.897904E-570.032
Produced by FastQC (version 0.11.5)