FastQCFastQC Report
Wed 25 May 2016
SRR1781107_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781107_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264078
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7005726.528904338869573No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGTA150.002223168369.9986832
TACTCGT150.002223168369.9986837
CGCGTGT150.002223168369.998687
CCGCCGT150.002223168369.9986822
TCGGTGT150.002223168369.9986831
TCGGTAC150.002223168369.9986867
CGAGTAC252.3878778E-455.9989450
GAATTCG252.3878778E-455.9989454
AGTCCGC200.00694665952.49900419
CTTTGCG200.00694665952.49900434
ACTCCGA200.00694665952.49900418
GTAGTCG200.00694665952.49900427
GATCGGG305.871681E-446.66578732
ACACCGA305.871681E-446.66578755
ACGAGTA305.871681E-446.66578749
TTCGAAC305.871681E-446.66578757
TCGAACA305.871681E-446.66578758
ATCGATT404.5856632E-543.7491743
GACTTAG350.001248340.037151
ATTAGAT350.001254129739.99924546
Produced by FastQC (version 0.11.5)