FastQCFastQC Report
Wed 25 May 2016
SRR1781107_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781107_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264078
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT4773018.074205348419785No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG46351.7551632472224115No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC21090.7986276781859906No Hit
ATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTT9800.371102477298374RNA PCR Primer, Index 14 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC8340.3158157817008611No Hit
CTTAAACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT7550.28590037791864525No Hit
CTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT7140.27037466203167243No Hit
CTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC6440.24386734222464576No Hit
CTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG3240.12269102310680936No Hit
CTTTACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC2740.10375722324464742No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCACATCTCGTATGCCGT2720.10299987125016094No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGA150.002223001370.000011
CGCAGAA150.002223001370.0000157
CGTAATC150.002223001370.0000148
AGGGGGG58900.065.0084970
GCCGTAT550.063.6363646
CGAATGC453.8380676E-1062.22222541
AACGTAT401.0630174E-861.2539
TAACGTA352.9671355E-760.00000438
ATATCCG352.9671355E-760.00000410
TGCCGTA700.060.00000445
ACCGGAA308.3623E-658.33333627
GCCGTCA1100.057.27272446
GCATGAA2400.056.87500456
GTTACCA1050.056.6666731
ACGTATG1050.056.6666740
TATGACG1550.056.4516143
ACCGGTA750.056.027
CGTATGA1750.056.041
CGTATAC252.3876541E-455.99999648
TCTACGA509.767973E-1055.99999611
Produced by FastQC (version 0.11.5)