FastQCFastQC Report
Wed 25 May 2016
SRR1781106_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781106_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences261732
Sequences flagged as poor quality0
Sequence length76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7906730.209145232527927No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAAT150.002223123669.99885649
CCGTGCG200.00694651952.499149
CACGAGT200.00694651952.4991446
CCGAAAC200.00694651952.4991434
CGAGAAT350.001254093639.99934814
CGATGTC400.002416226334.99942823
GACTAGG652.0953483E-532.313342
CTCGGTG552.9874733E-431.81766144
CTACTAG450.00428270431.140351
GTCCACC450.00428270431.140351
ACGATGT450.004302787631.11060122
TACGCCG450.004302787631.1106014
TACGATG450.004302787631.11060121
CGTGTCG450.004302787631.1106019
TCGGTGG450.004302787631.11060145
AGTACGT450.004302787631.1106015
GTACGTG450.004302787631.1106016
TACGTGT450.004302787631.1106017
GTATAAG703.4583987E-530.0281961
CCAGGAC604.974381E-429.166193
Produced by FastQC (version 0.11.5)