FastQCFastQC Report
Wed 25 May 2016
SRR1781106_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781106_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences261732
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT224148.563721669493987No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCGT50071.9130255375727843No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGCGTATGCCGT20670.7897391224611434No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCG14890.5689025415310318No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGG11240.4294469151651307No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGAGTATGCCGT11100.4240979322360277No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATTCCGT9450.361056347714456No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGGATGCCGT7410.2831140250332401No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCC6590.2517842678770651No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGGCGT5830.22274693197621995No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATTCCGT5520.2109027554903489No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCG5410.20669998318891078No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGCGTATGCCGG4720.18033713875261717No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCCT4540.17345987498662754No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTTTGCCGT4440.16963917289441108No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGCGGATGCCGT3920.14977152201488547No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGACGT3740.14289425824889582No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTAAGCCGT3640.13907355615667935No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCGG3430.1310500817630248No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGCGGATGCCGG3030.11576727339415892No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGACGT2910.11118243088349916No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCC2860.10927207983739093No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTGGTATGCCGT2850.10889000962816928No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGCGTATGCCG2680.10239481607140129No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGTA750.069.99274445
TAGCGTA150.002223894269.9927438
TCGGAAG150.002223894269.9927440
AATTCGC150.002223894269.9927427
TCGAGTT150.002223894269.9927438
CACGTAT550.069.9927439
GAATTCG150.002223894269.9927426
TTCGCGA150.002223894269.9927429
TAAGCGG150.002223894269.9927429
AGGGGGG46650.065.6416470
GACGTAT501.4551915E-1162.99346546
CTCGTTT900.062.2157739
CCGAATT453.8380676E-1062.2157747
CATCACG1300.061.91665636
TTGCCGG401.063745E-861.24365244
ACGTAAT750.060.66037847
CCGGATT1300.059.2246347
TCTTATG11500.059.03735450
TCTCGTT950.058.94125738
CTGCTTG13700.058.4975854
Produced by FastQC (version 0.11.5)