FastQCFastQC Report
Wed 25 May 2016
SRR1781105_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781105_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103493
Sequences flagged as poor quality0
Sequence length76
%GC85

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6874366.42284985457954No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAATG150.002214977370.033361
ATAGATT150.00221923169.999523
GGGTTTT150.00221923169.9995226
GCGGAGC150.00221923169.9995255
TGTCGGT150.00221923169.9995243
TGTCGGG150.00221923169.9995243
GTGTCGG308.326877E-658.3329342
GGGCTGC252.3809265E-455.9996115
ATATGAC200.00692119352.5250241
CCGGGGT200.006934446752.49963817
CATGATA200.006934446752.49963827
GAGTAAT200.006934446752.49963829
TCGGGCG200.006934446752.49963811
ACACAAC200.006934446752.49963850
AATGGTT200.006934446752.49963863
TATGACA200.006934446752.4996382
CGGGGAG200.006934446752.49963822
GGGGTTT200.006934446752.49963825
ATGACAT200.006934446752.4996383
GCGGCCG200.006934446752.49963817
Produced by FastQC (version 0.11.5)