FastQCFastQC Report
Wed 25 May 2016
SRR1781105_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781105_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103493
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT4631644.752785212526454No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG45544.400297604668914No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC22402.1643975921076786No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCCGT16311.5759519967534035No Hit
ATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTT9300.8986115002947058Illumina PCR Primer Index 11 (95% over 22bp)
CTTAAACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT8680.8387040669417256No Hit
CTAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT8200.7923241185394182No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC8180.790391620689322No Hit
CTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC5740.5546268829775927No Hit
CTTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG3300.31886214526586343No Hit
CTTTACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC2850.2753809436387002No Hit
CATATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT2790.2695834500884118No Hit
TTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC2320.22416975061115246No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC2120.204844772110191No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTCAATCTCGTATGCCGT2100.2029122742600949No Hit
TATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCT1940.18745229145932574Illumina PCR Primer Index 11 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG1910.18455354468418153No Hit
CTTATACACATCTCCGAGCCAACGAGACGACGCTCCATCTCGTATGCCGT1850.17875605113389312No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGACGT1710.1652285661832201No Hit
CTTATACACATCTCCGAGACCACGAGACGACGCTCCATCTCGTATGCCGT1440.13913984520692219No Hit
CCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG1420.13720734735682608No Hit
CTAAAACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT1380.1333423516566338No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCAGT1250.12078111563100886No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTACATCTCGTATGCCGT1200.1159498710057685No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1170.11305112423062429No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCCG1070.10338863498014358No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATGT150.002219169970.0000112
GAAGCTC150.002219169970.0000129
ACACGTA150.002219169970.0000138
GCCCAAG500.070.0000118
AATGACG150.002219169970.0000143
TACGAGA150.002219169970.0000113
AGTCTTA150.002219169970.0000148
CGAATGA150.002219169970.0000141
GAAAACG252.8227478E-670.0000118
CCGACTA150.002219169970.0000147
CGACTAC150.002219169970.0000148
CCTATAT150.002219169970.0000121
ATTGAGA150.002219169970.0000118
AACACGT150.002219169970.0000137
AGGGGGG54050.066.7622670
TGCATGA900.066.1111155
CGAATGC700.065.041
GCATGAA1250.064.456
CCAAGAG600.064.1666720
ACGCACC550.063.6363630
Produced by FastQC (version 0.11.5)