FastQCFastQC Report
Wed 25 May 2016
SRR1781104_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781104_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163923
Sequences flagged as poor quality0
Sequence length76
%GC67

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6129937.39499643125126No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGA150.002197847570.189051
CGTATCA252.8310114E-669.9963456
ATGTACG150.002221953869.9963458
CCGTATC252.8310114E-669.9963455
GCGTCGG150.002221953869.996348
GCCGTAT252.8310114E-669.9963454
ACGCGTC150.002221953869.996346
TGGTCGC308.350798E-658.33028449
CGGTGGT553.6379788E-1157.26973346
GTATTCG200.006867785552.641791
TTGGGCG200.00691779152.5453452
CAGGTCC200.006942895252.49725335
CCGGGTG200.006942895252.4972535
ATAGGCG200.006942895252.49725343
CGCCTAA200.006942895252.49725334
CGGCTGG200.006942895252.4972539
GGCATCA200.006942895252.49725329
CGCGTCG200.006942895252.4972537
TCGCCGT352.0802187E-549.99738752
GTAGATC352.0802187E-549.99738738
Produced by FastQC (version 0.11.5)