FastQCFastQC Report
Wed 25 May 2016
SRR1781104_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781104_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163923
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT4222825.760875533024652No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG40102.446270505054202No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC18971.157250660371028No Hit
ATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCTT9370.5716098412059321TruSeq Adapter, Index 9 (95% over 21bp)
CTAATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT9140.5575788632467683No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC7390.45082142225313104No Hit
CTTAAACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT7370.4496013372132038No Hit
CTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTC5710.3483342788992393No Hit
CATATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT3400.2074144567876381No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGAAAGATCTCGTATGCCGT2930.17874245834934696No Hit
CTTTACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTC2830.17264203314971055No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG2370.14458007723138303No Hit
TTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTC2260.13786960951178295No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGCTCTCGTATGCCGT2100.12810892919236472No Hit
CTTATACACATCTCCGAGCCAACGAGACGGAGTAAGATCTCGTATGCCGT1970.12017837643283737No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC1950.11895829139291009No Hit
CTTAATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG1870.11407795123320094No Hit
TATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCT1790.10919761107349181No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAATCTCGTATGCCGTC1710.1043172709137827No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAC150.002221492870.000013
CCGATTG150.002221492870.0000115
CGAAAGC207.908384E-570.041
CCGTATT252.8301329E-670.047
AATGCCG550.070.043
CTCGAAA207.908384E-570.039
TGCCGAC252.8301329E-670.045
AGGGGGG52250.065.7129270
ACCGAGC700.065.013
GAATGCC650.064.6153942
ACTCGTA600.064.1666738
AACTCGT600.064.1666737
CGACTTC501.4551915E-1162.99999648
TCACCGA850.061.76470611
CGAATGC800.061.2541
AACGAGC401.0595613E-861.2513
GCCGTAT401.0595613E-861.2546
CGAGCAC1450.060.34482615
TAAACAC3600.060.2777753
CGTCTTA352.9601324E-760.048
Produced by FastQC (version 0.11.5)