FastQCFastQC Report
Wed 25 May 2016
SRR1781103_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781103_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205420
Sequences flagged as poor quality0
Sequence length76
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7066334.399279524875865No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGAT150.002222327569.99975636
TACCAGA252.3864416E-455.99980515
GTATAGC252.3864416E-455.9998053
TCGCCAG200.006944046452.49981736
TCGTTTA200.006944046452.49981722
CGTATCA200.006944046452.49981756
GGGCCGT200.006944046452.4998176
CGGACTA200.006944046452.49981751
CGCGTGT350.001253384439.999867
TTGTGCG459.1757174E-538.8887562
CCGGCGA459.1757174E-538.88875621
GCGAGAA400.002414866834.99987813
CTAACTC400.002414866834.9998783
TATTCCA400.002414866834.9998785
CCATACT400.002414866834.99987828
CGGTGGT601.2117263E-534.99987846
TATGTTA400.002414866834.99987844
TCTATAC552.9850245E-431.8180713
GATGTAT450.0043003831.11100424
TAATCTC450.0043003831.11100410
Produced by FastQC (version 0.11.5)