FastQCFastQC Report
Wed 25 May 2016
SRR1781103_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781103_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205420
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT4631922.548437347872653No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG47682.321098237756791No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC22041.0729237659429463No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTAGTATGCCGT13290.6469671891734008No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC7870.3831175153344368No Hit
ATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTCTT7300.35536948690487785TruSeq Adapter, Index 2 (95% over 21bp)
CTTAAACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT5860.28526920455651833No Hit
CTAATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT5400.26287605880634796No Hit
CTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTC5010.24389056567033396No Hit
CTTATACACATCTCCGAGCCCACGAGACAATCCTGTATCTCGTATGCCGT4940.2404829130561776No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGACGT3250.15821244280011684No Hit
CTTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG2990.14555544737610748No Hit
CTTTACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTC2470.1202414565280888No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTAAGCCGT2370.11537338136500827No Hit
TTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTC2080.10125596339207478No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTTA150.002223185769.9929448
AGGGGGG51050.065.6740870
TAAGCCG1300.061.91683243
TCGAATG750.060.6605540
ACTCCGA352.9609328E-760.00855611
ATATCGT352.965935E-759.99394637
AAGCCGT1350.059.62361544
GTATTCT1300.059.22479649
GCCGTAA900.058.3274546
TACGAGC553.6379788E-1157.26695313
TGCCGTA2450.057.13709645
CGTAAGC1300.056.53275741
TCGTATG65600.056.22908840
GCCGTCT62050.056.1748446
CGTATGC64750.056.15649841
CTCGTAT65700.056.14349739
TCTCGTA66650.056.13087538
TATCTCG67950.056.08706336
CGTCTTA6750.055.9943548
ATCGTAA252.3875902E-455.9943539
Produced by FastQC (version 0.11.5)