FastQCFastQC Report
Wed 25 May 2016
SRR1781102_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781102_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177394
Sequences flagged as poor quality0
Sequence length76
%GC71

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7748043.676787264507254No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGT207.910802E-569.998885
CGCGTCT150.002221936669.998877
TGGCGGC252.3856982E-455.999119
TGGCGCT252.3856982E-455.999113
GCGCGTC200.006942836652.4991576
GGGCGAG200.006942836652.49915722
AGCGCCG200.006942836652.49915762
CCGCGCG200.006942836652.4991573
TCTGGCG200.006942836652.49915711
CCTTGAT200.006942836652.4991572
TGTGCGC305.8663555E-446.6659163
GGCGCTG404.579463E-543.749314
CGGTGGT503.464589E-641.99932546
CGTGGGT350.001252999239.9993558
GTGCGCG350.001252999239.9993554
TCGGTGG701.9128493E-839.99935545
GCCGGCG556.6679713E-638.18120620
TGCATGT450.004299076331.11061128
CTAGTTC450.004299076331.1106113
AAGCTTT450.004299076331.11061140
Produced by FastQC (version 0.11.5)