FastQCFastQC Report
Wed 25 May 2016
SRR1781102_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781102_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177394
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT5295229.849938554855292No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG54133.0513997091220673No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC28141.5862994238813037No Hit
CTAATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT11140.6279806532351715RNA PCR Primer, Index 19 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCTT10700.6031771085831539No Hit
CTTAAACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT9440.5321487761705582No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC8530.4808505360947946No Hit
CTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTC6800.3833275082584529RNA PCR Primer, Index 19 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAATCTCGTATGCCGTC4870.27453014194392145No Hit
CATATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT3310.18659030181404104RNA PCR Primer, Index 19 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGCGATAAAATCTCGTATGCCGT3280.1848991510423126No Hit
CTTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG3220.18151684949885566No Hit
CTTTACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTC2870.1617867571620235No Hit
CTTAATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG2400.13529206173827749No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTC2380.13416462789045852RNA PCR Primer, Index 19 (95% over 22bp)
TATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCT2200.12401772326008773No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC2160.12176285556444975No Hit
CTTATACACATCTCCGAGCCAACGAGACGCGTTAAAATCTCGTATGCCGT2080.11725312017317384RNA PCR Primer, Index 19 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGCGTAAAAATCTCGTATGCCGT2070.11668940324926434No Hit
CCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG1920.10823364939062201No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGAG207.910174E-570.012
AGCCGAC150.002221794870.045
AAATCGA150.002221794870.037
CTCGTTA150.002221794870.028
TCCTCGC150.002221794870.068
CGTCTAA150.002221794870.048
TACGGTG150.002221794870.028
CCGTCAA150.002221794870.047
GTAATAC150.002221794870.03
CGTTAAC207.910174E-570.030
TAACCGA150.002221794870.011
GCCGACA207.910174E-570.046
ACGTCTA150.002221794870.047
CGAGACT252.8310915E-670.023
ATAACCG150.002221794870.010
GGTCTAT150.002221794870.031
GACGACT150.002221794870.046
TAAGACG150.002221794870.043
GCCCGTA150.002221794870.055
AGGGGGG66450.066.78705670
Produced by FastQC (version 0.11.5)