FastQCFastQC Report
Wed 25 May 2016
SRR1781101_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781101_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461560
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8574918.57808302279227No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTTT150.002224195569.9989229
GTTCGAC200.00694983952.49918721
TTAACCG200.00694983952.49918743
GCCGTAT305.8761763E-446.66594354
CGTGCGA459.1941794E-538.88828710
CCGTGCG459.1941794E-538.8882879
TGGTCGC753.534842E-837.33275649
TGCGGGT501.7090568E-434.999465
CCGAAAT501.7090568E-434.9994647
TCGCCGT552.9909716E-431.81768852
TCCGTGC703.4867353E-529.9995358
CGTATCA500.007206515427.99956756
TCTTCGA500.007206515427.99956737
TAGCCGC500.007206515427.9995674
CGCCGTA657.9528004E-426.92265953
GCGAGAA808.6605534E-526.24959413
CGAGAAT808.6605534E-526.24959414
AGGTCGC808.6605534E-526.24959435
ACCGAAA851.3074998E-424.705546
AGTGGTC851.3074998E-424.705562
Produced by FastQC (version 0.11.5)