FastQCFastQC Report
Wed 25 May 2016
SRR1781101_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781101_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461560
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT5732012.418753791489731No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG57431.2442586012652743No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC28180.610538174885172No Hit
ATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTT14220.308085622670942No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC11730.2541381402201231No Hit
CTAATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT11110.24070543374642514No Hit
CTTAAACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT10490.22727272727272727No Hit
CTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC8870.19217436519629083No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAATAAATCTCGTATGCCGT7270.15750931623190917No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAC150.002224549769.996112
AGCTTCG150.002224549769.996124
AGGGGGG69300.063.5318170
TGCCGTA501.4551915E-1162.99648745
CGTCTAC950.055.2600848
TATGCCG86200.054.4053243
ATGCCGT86450.054.3694444
CGTATGC87500.054.2369841
TGCCGTC86200.054.16171645
GTATGCC86700.054.13192742
CTCGTAT87250.054.11160339
GCCGTCT86150.054.07127446
TCGTATG88500.054.05913540
TCTCGTA87150.054.05321538
AATCTCG87300.054.04051636
CCGTCTT85650.053.97831347
CGTCTTC86000.053.9621148
ATCTCGT86700.053.7282637
CTGCTTG87200.053.66100354
TAAATCT87450.053.5876434