FastQCFastQC Report
Wed 25 May 2016
SRR1781100_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781100_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences667327
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7690311.524035442893814No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCGA150.002221898470.0206151
CAGGTAT806.2284016E-835.0103071
GCCGTAT400.002418771934.99981354
TCGGTGG552.9922137E-431.81801245
GCGCTGG450.00430729131.11094715
CCCCGTA500.00720842527.9998519
CCGACGT808.665639E-526.24985966
CACGAGG959.390533E-625.78933314
TCCTGGA7150.025.45440970
CGGCTGG700.001226053624.9998669
TGCCTAC1551.2878445E-924.83857745
CAACCGT851.3082636E-424.705756
GTATCGG851.3082636E-424.7057511
TATCCGG851.3082636E-424.7057553
TAAACGT1001.3976778E-524.4998684
TGCCCCG901.9277625E-423.3332162
CGCGGTC901.9277625E-423.3332144
CGACGTT901.9277625E-423.3332167
CCGGAAT901.9277625E-423.3332156
TTGTACT1802.8558134E-1023.333214
Produced by FastQC (version 0.11.5)