FastQCFastQC Report
Wed 25 May 2016
SRR1781100_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781100_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences667327
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT518637.77175207956519No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG51430.7706866348881433No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC21680.32487820813484247No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC10140.1519494940261671No Hit
ATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCTT9940.14895246258580874TruSeq Adapter, Index 7 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC7830.11733378089002842No Hit
CTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT6980.10459639726850553No Hit
CTTAAACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT6900.10339758469236221No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAATT207.928115E-569.9965547
TAAGACG150.00222492969.9965543
AGGGGGG62100.062.5009570
AACGTAT401.0666554E-861.24698639
CCGACTT401.0666554E-861.24698647
CACGTAT1150.060.8665739
ACGTATG1400.059.99704440
TATGCCG75200.056.872243
ATCGTAT800.056.872239
CGTCTTC68400.056.84661548
CGTATGC74300.056.76032341
GCCGTCT71050.056.74597546
CCGTCTT70950.056.62864347
TCTTCTG67950.056.45049750
TCGTATG74350.056.4397240
TCTCGTA74400.056.3547538
CATCTCG75250.056.32280736
CTCGTAT74100.056.2995239
TGCCGTC74050.056.2430145
ATGCCGT76100.056.1536144