FastQCFastQC Report
Wed 25 May 2016
SRR1781099_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781099_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238417
Sequences flagged as poor quality0
Sequence length76
%GC70

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9352839.22874627228763No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGAG150.002222762469.99979413
CGTTAAC150.002222762469.99979410
ACCGTTA150.002222762469.9997948
TGCACGG150.002222762469.99979447
AAGCGCA252.387217E-455.99983248
AGACGTT252.387217E-455.99983268
GGTGTCG200.006945396352.49984441
ACGTTAG200.006945396352.49984470
GCACGGT200.006945396352.49984448
TACGCGT200.006945396352.4998445
GGTCTAA305.863998E-446.676321
GTAAGCG305.870066E-446.66652746
TATACCG305.870066E-446.6665276
ATACCGC305.870066E-446.6665277
TATACGC404.5838828E-543.749873
TTATACG350.001253786539.999882
GTGTCGG350.001253786539.9998842
GTCGTGT350.001253786539.9998812
GCGCTGC556.677039E-638.18170515
GTCCTAC400.002413152335.0072361
Produced by FastQC (version 0.11.5)