FastQCFastQC Report
Wed 25 May 2016
SRR1781099_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781099_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238417
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT6402726.855048088013856No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG64952.7242184911310856No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC34001.4260728052110379No Hit
ATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCTT14490.607758674926704RNA PCR Primer, Index 19 (95% over 23bp)
CTAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT13860.5813343847124995No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC12690.5322607028861197No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT11680.48989795190779184No Hit
CTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC9210.38629795694098995No Hit
CATATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT5130.2151692203156654No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG4740.1988113263735388No Hit
CTTTACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC3810.15980404081923688RNA PCR Primer, Index 19 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG3190.1337991837830356No Hit
CTTATACACATCTCCGAGCCCACGAGACTTATGAAATCTCGTATGCCGTC2970.12457165386696419No Hit
TTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC2930.1228939211549512No Hit
CTTATACACATCTCCGAGCCCACGAGACTAATGAAAATCTCGTATGCCGT2910.12205505479894471No Hit
CTTATACACATCTCCGAGCCAACGAGACTTATGAAAATCTCGTATGCCGT2830.11869958937491874No Hit
TATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCT2650.1111497921708603RNA PCR Primer, Index 19 (95% over 22bp)
TCTTAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC2590.10863319310284084No Hit
CCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG2560.10737489356883109No Hit
CTTATACACATCTCCGAGCCCACGAGACATATGAAAATCTCGTATGCCGT2430.10192226225478888No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGC150.002219382270.026653
AATCACG301.0210897E-769.9972836
TTCGTGG150.00222307969.9972816
GAGAGTC150.00222307969.9972823
CGTAGTC150.00222307969.9972827
TACGAGA252.8347422E-669.9972813
GCCGTAT301.0210897E-769.9972846
ACGACTA150.00222307969.9972847
ATGACGA207.9172816E-569.9972744
GACGACT353.6980055E-969.9972746
AGGGGGG76150.066.6421970
ACGTATG501.4551915E-1162.99754740
AACTCGT900.062.21979537
TGCCGTA453.8380676E-1062.21979545
CGTCTAC1250.061.59760348
CACGTAT401.0626536E-861.24761239
ATCGTAA352.966699E-759.9976639
AAACTCG1000.059.4976836
AAGCCGT1000.059.4976844
CGTATGA1800.058.3310641
Produced by FastQC (version 0.11.5)