FastQCFastQC Report
Wed 25 May 2016
SRR1781098_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781098_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences289026
Sequences flagged as poor quality0
Sequence length76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8631129.862711313169054No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT150.002223476569.99792522
GAGACCG252.3883834E-455.9983375
AGCGTAG200.00694761352.49844743
CGAGTTG200.00694761352.49844747
CGACGAG200.00694761352.49844722
TTGGCGT305.8729196E-446.66528338
ATTGGCG305.8729196E-446.66528337
GCGTGAA305.8729196E-446.66528341
CGTGAAG350.001254392639.99881742
TGGCGTG350.001254392639.99881739
TATCGCT350.001254392639.99881719
CGGTGGT1250.039.19883746
GTCTCGG400.00241679934.99896242
CGCGTCT400.00241679934.9989627
GCCGTAT806.2251274E-834.99896254
GTATCAT851.06059815E-732.940257
GTGGTCG1109.0221874E-1031.81723648
TCGGTGG1450.031.3783845
CGTGCCT450.004303802331.1101963
CCGTATC802.4607434E-630.62409455
Produced by FastQC (version 0.11.5)