FastQCFastQC Report
Wed 25 May 2016
SRR1781098_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781098_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences289026
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT5709919.755662120362874No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG82942.869638025644752No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC31451.088137399403514No Hit
ATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTT16460.5694989378118231Illumina PCR Primer Index 3 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC16380.5667310207386187No Hit
ATTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT11480.39719610000484384No Hit
CTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTC11370.39339021402918767No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG5790.20032799817317473No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTC4700.16261512805076359No Hit
TTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTC4450.15396538719699956No Hit
CTTATACACATCTCCGAGCCAACGAGACTTTAATGCATCTCGTATGCCGT4040.13977981219682659No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC3930.1359739262211704No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3790.13113007134306257No Hit
CATATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT3770.13043809207476142No Hit
TATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCT3330.11521454817213676No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTAATGAATCTCGTATGCCGT3330.11521454817213676No Hit
CTTATACACATCTCCGAGCCCACGAGACTATAATGCATCTCGTATGCCGT3270.1131386103672334No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG3200.11071668292817946No Hit
CTTATACACATCTCCGAGACCACGAGACTTTAATGCATCTCGTATGCCGT3080.10656480731837276No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCG150.002223737869.9958567
TCACGTA252.836612E-669.9958438
AGGGGGG72850.064.8554570
CACGTAT308.367235E-658.32987239
ATATCCG1050.056.66330310
TATGACG1450.053.10029643
CTTATCG200.00694842952.49688746
AACGCGC200.00694842952.49688768
CGTTTAC200.00694842952.49688714
TCTTTAT2350.052.1606671
GCCGTCT92650.052.05303646
CATCTCG93950.051.92880236
CTTGAAA95100.051.92646857
TATGCCG94050.051.91079743
ATGCCGT94050.051.91079744
CCGTCTT92650.051.9019447
TCTCGTA94900.051.88838638
CGTCTTC92550.051.88238548
TGCCGTC93700.051.88059645
CGTATGA1350.051.84877841
Produced by FastQC (version 0.11.5)