FastQCFastQC Report
Wed 25 May 2016
SRR1781097_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781097_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331137
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7808323.580270401676646No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCGG200.006948103752.49920742
GGGCCGT305.873745E-446.6659626
GTCGCCG602.592642E-740.83271851
TCGCCGT556.685468E-638.1812452
GCCGTAT556.685468E-638.1812454
CGTATCA654.85672E-737.6917456
GCACTAC501.7080108E-434.99947418
TCAACCG400.00241713434.9994737
TCGGTGG957.610652E-933.15739445
GGCGGCA450.00428533731.1388551
CGGTGGT901.7525417E-731.11064146
TGCACTA450.00430439331.11064117
CCCGCTA450.00430439331.11064117
CGCCGTA703.4837787E-529.99954853
AGCGTTC604.977163E-429.1662278
GGTCCTA755.5424585E-528.024971
CTCGGTG906.1589E-627.22181144
TGGTCGC906.1589E-627.22181149
GGCCTAT657.9060177E-426.9470861
CCGTATC808.653278E-526.24960355
Produced by FastQC (version 0.11.5)