FastQCFastQC Report
Wed 25 May 2016
SRR1781097_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781097_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331137
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT5109515.430169386084913No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG74882.2612997037479956No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC24580.7422909551031748No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC13400.40466634655746714No Hit
ATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCTT12340.37265542660590634RNA PCR Primer, Index 34 (95% over 23bp)
ATTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT9800.29595001464650583No Hit
CTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC9490.2865883305097286No Hit
CTTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG4320.1304595982931536No Hit
CTTTACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC4160.1256277613193331RNA PCR Primer, Index 34 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC3970.11988995491292123No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTAGTATGCCGT3840.11596408737169209No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATATCGTATGCCGT3760.11354816888478182No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC3620.1093203115326889No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCATA301.0221265E-769.999148
AGGGGGG60200.063.6619670
TGACGAC453.8380676E-1062.22141645
TCTACGA900.062.22141611
GCCGTAA453.8380676E-1062.22141646
ATATCGT1800.058.3325837
GCCGACT601.8189894E-1258.3325846
ATCGTAT1900.057.10452739
GCAGCGT252.386914E-456.0077321
ATCTCGA750.055.9992737
ATCTACG1000.055.9992710
AAGCCGT750.055.9992744
TACGAGA252.3886962E-455.9992713
CCGACTT509.786163E-1055.9992747
CGACTTC509.786163E-1055.9992748
ACGACAT252.3886962E-455.9992747
GCCGTCA2400.053.95763846
CGTAAGC1200.052.49932541
TATCGTA2000.052.49931738
TCGTATG81800.051.77194640
Produced by FastQC (version 0.11.5)