FastQCFastQC Report
Wed 25 May 2016
SRR1781096_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781096_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222788
Sequences flagged as poor quality0
Sequence length76
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7578334.01574591091082No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC150.002222685169.9988833
CGCGTTA150.002222685169.9988840
ATCGCGT150.002222685169.9988838
CGGTGGT509.767973E-1055.99910446
CGGCGAA200.00694515852.4991648
GATCGCG200.00694515852.4991637
TGGACCG200.00694515852.4991651
CGTTCGC200.00694515852.4991649
CGTTATT200.00694515852.4991642
TACGAAT200.00694515852.4991638
ATACGAA200.00694515852.4991637
CGATCGC200.00694515852.4991636
TCGCGTT200.00694515852.4991639
GACTCGG200.00694515852.4991666
CGTATCA352.0822059E-549.999256
ACTCGAT305.869609E-446.6659267
TACTCGA305.869609E-446.6659266
CTCGATT305.869609E-446.6659268
CGCCGTA404.5832603E-543.74930253
TCGCCGT503.4686018E-641.9993352
Produced by FastQC (version 0.11.5)