FastQCFastQC Report
Wed 25 May 2016
SRR1781096_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781096_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222788
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT4984422.372838752536044No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG70573.1675853277555346No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC24811.1136147368799036No Hit
ATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTT12370.5552363682065462TruSeq Adapter, Index 10 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC11880.5332423649388657No Hit
ATTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT10740.4820726430507927No Hit
CTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC8190.36761405461694524No Hit
CTTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG4560.20467888755229188No Hit
TTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC3720.1669748819505539No Hit
CATATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT3710.1665260247410094No Hit
CTTATACACATCTCCGAGCCAACGAGACACCGGCTAATCTCGTATGCCGT3590.1611397382264754No Hit
CTTATACACATCTCCGAGCCCACGAGACACAGGCTAATCTCGTATGCCGT3580.1606908810169309No Hit
CTTTACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC3210.1440831642637844No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3150.1413900210065174No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATATCGTATGCCGT3050.13690144891107242No Hit
TCTTAATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC2970.13331059123471642No Hit
CTTATACACATCTCCGAGACCACGAGACACCGGCTAATCTCGTATGCCGT2710.12164030378655942No Hit
TATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCT2540.11400973122430293No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCTATCTCGTATGCCGTC2500.11221430238612493No Hit
CTTAATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG2400.10772573029067993No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGACGT2330.10458372982386843No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAT150.00222291169.99708639
ATTAGGT150.00222291169.99708659
CGCGATC150.00222291169.99708640
TCGATAG150.00222291169.99708645
TCGTCTT150.00222291169.99708614
CGTCAAC207.916248E-569.99708648
CGGATAA800.065.6222731
AGGGGGG61000.065.1202370
ACCGGAT750.060.66413529
CCGTCAA308.360088E-658.33090247
ATATCGT1650.057.2703437
TATCGTA1650.057.2703438
AAACTCG800.056.8726336
AAGCCGT800.056.8726344
AACTCGT800.056.8726337
ATCGTAT1850.056.7543939
ACGTAAG252.3873312E-455.99766540
AGCCGTC750.055.9976645
AATCACG750.055.9976636
TCTCGAA900.054.44217738
Produced by FastQC (version 0.11.5)