FastQCFastQC Report
Wed 25 May 2016
SRR1781095_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781095_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197749
Sequences flagged as poor quality0
Sequence length76
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8395142.453312026862335No Hit
CTTATACACATCTGACGCTGCCGACGACGCAAATAGTGTAGATCTCGGTG2040.10316107793212609No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTAC150.00221561970.052131
TCGCCGG252.386351E-455.9991952
GTCTCAC200.006923146552.5391041
GTGTTTG200.00694396552.49924562
CTAAGTT200.00694396552.49924570
TGCGGGT200.00694396552.4992455
CGATAAA200.00694396552.49924525
CTGGCGC407.453327E-752.49924512
TCTGGCG407.453327E-752.49924511
TGCCGGC200.00694396552.49924519
TCGCCGT750.051.33259252
GTCGCCG1050.049.9992851
CGCCGTA707.2759576E-1249.99927553
CGTATCA651.8371793E-1048.4608456
TGGTCGC1100.047.7265849
TGCGCGG305.867953E-446.6659935
GCCGTAT751.4551915E-1146.66599354
GTGGTCG1300.045.7685748
CGGTGGT1250.044.7993546
TGGCGCT551.3053977E-744.5448113
Produced by FastQC (version 0.11.5)