FastQCFastQC Report
Wed 25 May 2016
SRR1781095_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781095_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197749
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT5423627.426687366307796No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG79764.0333958705227335No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC26101.319854967661025No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC14010.7084738734456306No Hit
ATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTT13550.6852120617550531RNA PCR Primer, Index 14 (95% over 21bp)
ATTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT10230.5173224643361028No Hit
CTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC9650.4879923539436356No Hit
CTTTACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC4760.24070918184162754No Hit
CTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG4700.23767503249068261No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCACATCTCGTATGCCGT4160.2103676883321787No Hit
TTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC3900.1972197078114175No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGTTCCCATCTCGTATGCCGT3590.18154326949820226No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTACCATCTCGTATGCCGT3490.17648635391329412No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGACGT3470.1754749707963125No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCAATCTCGTATGCCGT3290.16637252274347786No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3240.16384406495102377No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC3210.16232699027555134No Hit
CTTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG3180.1608099156000789No Hit
CTTATACACATCTCCGAGCCAACGAGACCGGTTCCCATCTCGTATGCCGT2920.1476619350793177No Hit
CATATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT2800.14159363637742797No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGATCCCATCTCGTATGCCGT2800.14159363637742797No Hit
CCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG2790.14108794481893713No Hit
TATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCT2690.136031029234029No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCAGT2440.12338874027175864No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATATCGTATGCCGT2110.10670091884156178No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCATCTCGTATGCCGTC1990.10063262013967202No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGTC150.00221622270.0473257
ATGACGA252.8336926E-669.9941944
CTTAAGC150.002222905669.9941851
AGCCGTA207.915672E-569.9941845
ATCTCGC150.002222905669.9941811
AAAACCG150.002222905669.9941810
TGCTCAT150.002222905669.9941810
CCGACTT600.069.9941847
CCCGGAA150.002222905669.9941825
TCGAAGT150.002222905669.9941835
ATTCTCG150.002222905669.9941869
AGGGGGG66800.065.4361770
CGAGCAC600.064.1613315
GTTCACA1800.064.1613331
CGGTTCA1500.062.99476629
GTTACCA1000.062.99476631
TATGACG1800.062.2170543
CGACTTC750.060.66162548
ACGGTTC750.060.66162528
GTATGAC1850.060.5355142
Produced by FastQC (version 0.11.5)