FastQCFastQC Report
Wed 25 May 2016
SRR1781094_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781094_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193168
Sequences flagged as poor quality0
Sequence length76
%GC70

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7677339.74416052348215No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCAG150.002222160869.9994841
TCGTTCG150.002222160869.9994823
CGTTCGG150.002222160869.9994824
GTACTTA150.002222160869.9994852
TCGGTAA150.002222160869.9994827
GCTATAC200.006936423552.5132031
AACGTCG200.006943531352.4996126
CGAGTAA200.006943531352.4996121
CCGGACA200.006943531352.499614
TTCGTTC200.006943531352.4996122
CCTGTGC200.006943531352.4996154
TACGCAA200.006943531352.4996137
TTCGGTA200.006943531352.4996126
CGGTCTC200.006943531352.4996162
CGCACAG200.006943531352.499615
CGCCGTA552.2700988E-950.90871453
GCCGTAT552.2700988E-950.90871454
TGGTGAC352.0810605E-549.9996343
TCGCCGT651.8371793E-1048.46117852
GTATCAT651.8371793E-1048.46117857
Produced by FastQC (version 0.11.5)