FastQCFastQC Report
Wed 25 May 2016
SRR1781094_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781094_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193168
Sequences flagged as poor quality0
Sequence length76
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT188279.746438333471383No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCGT49832.5796198128054337No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCG16090.8329536983351279No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGCGTATGCCGT15820.8189762279466578No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGAGTATGCCGT9920.5135426157541622No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATTCCGT8710.4509028410502775No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGG8410.43537231839642176No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCG7190.3722148596040752No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGGATGCCGT6370.3297647643502029No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATTCCGT5900.30543361219249565No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCCT5710.2955976145117204No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGGCGT5390.2790317236809409No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCC5320.27540793506170796No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTAAGCCGT4710.24382920566553465No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGACGT4600.23813468069245422No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGCGTATGCCGG3990.20655595129628096No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGACGT3720.19257848090781082No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGCGTATGCCG3670.18999006046550151No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTTTGCCGT3280.1698003810154891No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCGG3160.16358817195394681No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCC3150.16307048786548495No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGCGGATGCCGT3050.1578936469808664No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTGGTATGCCGT2680.1387393357077777No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGAGTATGCCG2670.13822165161931582No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCCT2510.1299387062039261No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2500.12942102211546427No Hit
CTTATACACATCTCCGAGCCCACGAGACATCGCGGCATCTCGTATGCCGT2380.12320881305392198No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGCGGATGCCGG2340.12113807670007454No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGGCGT2210.11440818355007042No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGAGTATGCCGG2180.11285513128468483No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTAAGCCGT2080.10767829040006627No Hit
CTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGTC1980.10250144951544769No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCACGTATGCCGT1960.10146608133852397No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGA1950.10094839725006213No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCT150.0022201470.015534
GCATAAC207.913404E-569.9974135
ACAACGC150.002222421669.9974127
CGTATAA150.002222421669.9974148
TCGGGTT207.913404E-569.9974139
TACGGAA150.002222421669.9974146
ATCACGG301.0200347E-769.9974137
TTGCGGG207.913404E-569.9974145
CGTAAAC150.002222421669.9974148
TTCGCGT150.002222421669.9974129
GCGAGTG150.002222421669.9974133
CGAATGC800.065.62257441
AGGGGGG43150.065.2930770
TACGCGG550.063.63401429
CCGAAAT850.061.7624247
GATGCCG3750.061.5977243
GCCGACT401.0611984E-861.24773446
CCGACTT401.0611984E-861.24773447
AAGCTTT800.061.24773454
GCCGTAT8250.061.08865446
Produced by FastQC (version 0.11.5)