FastQCFastQC Report
Wed 25 May 2016
SRR1781093_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781093_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168141
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3121918.56715494733587No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGATAC150.002221779769.9985148
CGACGAT150.002221779769.9985122
GCAACCG150.002221779769.9985169
CGCAAGC150.002221779769.9985164
TGGGAAA150.002221779769.9985128
CTCGGTG252.3853999E-455.99880644
CTATGGG200.00693418552.5145032
TGATACC200.00694235152.49888649
CAACGCA200.00694235152.49888670
ACGCAAG200.00694235152.49888663
CACGCAA200.00694235152.49888662
GAATAGA200.00694235152.49888618
GGCCAGT200.00694235152.4988867
AGTTCCT200.00694235152.49888662
TTGTCTT352.0800668E-549.99893650
GTAGATC352.0800668E-549.99893638
AGATCTC451.6773974E-646.6656840
GCACCTG305.865626E-446.66567611
GCCAGCA305.865626E-446.66567614
TACAGCG305.865626E-446.66567666
Produced by FastQC (version 0.11.5)