FastQCFastQC Report
Wed 25 May 2016
SRR1781093_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781093_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168141
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT164949.809624065516442No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG30561.817522198630911No Hit
ATTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT8500.5055280984411893No Hit
ATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTT7820.4650858505658941Illumina PCR Primer Index 11 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC5740.34138015118263837No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC5630.3348380228498701No Hit
CTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC5280.3140221599728799No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCCGT4960.2949905139139175No Hit
CTTTACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC2860.17009533665197663No Hit
TTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC1970.1171635710504874No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC1700.10110561968823785No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAC150.002221592570.025
CACGGAA150.002221592570.058
ACTGAGG150.002221592570.08
GACACGA150.002221592570.024
GCAACTT150.002221592570.01
TTACTGA150.002221592570.06
GCCGACG150.002221592570.026
ACGGAAG150.002221592570.059
CCGACGC150.002221592570.027
GAACACG150.002221592570.018
AGGGCTT150.002221592570.012
TAAGACG150.002221592570.043
CGTCTAC600.064.16666448
GACGATC401.0597432E-861.2529
TCACCGA352.9606053E-760.011
CGACGAT352.9606053E-760.028
GCCGTAT352.9606053E-760.046
GTATGAC1050.059.99999642
AGGGGGG24450.059.6932570
TATGACG950.058.94736543
Produced by FastQC (version 0.11.5)