FastQCFastQC Report
Wed 25 May 2016
SRR1781092_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781092_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222073
Sequences flagged as poor quality0
Sequence length76
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7707334.706155183205524No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGA150.002222761569.998242
ACCCGTC252.3871278E-455.99855440
GTGGTCG653.6379788E-1253.8447748
TCGCCGT407.458111E-752.4986552
CGTATCA407.458111E-752.4986556
CGAAAGT200.006945397252.4986543
GCGCGCT200.006945397252.4986553
CGGTTGG200.006945397252.4986525
CCCGTCG200.006945397252.4986541
TGCGCGT800.052.498655
GGCGGTT200.006945397252.4986523
CGGGAAC200.006945397252.4986535
CGCCGTA407.458111E-752.4986553
CTCGGTG651.8371793E-1048.46029344
TGTGCGC800.048.123763
CGCTGCG305.8698497E-446.66546616
TGGTCGC604.9112714E-946.66546649
TGCGGGC451.6799058E-646.66546219
CGCGTCT451.6799058E-646.6654627
CGGTGGT851.8189894E-1245.2929546
Produced by FastQC (version 0.11.5)