FastQCFastQC Report
Wed 25 May 2016
SRR1781092_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781092_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222073
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT5159323.232450590571567No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG74293.3452963665101114No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC25711.1577274139584732No Hit
ATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCTT14010.6308736316436487TruSeq Adapter, Index 9 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC13240.5962003485340406No Hit
ATTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT12240.5511701107293547No Hit
CTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTC9050.40752365213240693No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG4220.19002760353577428No Hit
TTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTC4040.18192216073093084No Hit
CTTTACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTC4020.1810215559748371No Hit
CATATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT3910.17606822981632164No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGAAAGATCTCGTATGCCGT3900.17561792743827478No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGCTCTCGTATGCCGT3210.14454706335304157No Hit
CTTATACACATCTCCGAGCCAACGAGACGGAGTAAGATCTCGTATGCCGT3170.14274585384085414No Hit
CTTATACACATCTCCGAGACCACGAGACGGAGTAAGATCTCGTATGCCGT3100.1395937371945261No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3060.13779252768233868No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC2990.13464041103601068No Hit
TATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCT2860.1287864801214015No Hit
CTTAATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG2690.12113133969460493No Hit
CTTATACACATCTCAGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT2650.11933013018241749No Hit
CTAATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT2480.1116749897556209No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGACGT2260.10176833743859003No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG207.9155696E-569.998223
CGTCTTA600.069.998248
ACTCGAA301.0206713E-769.998238
AACACGA150.002222761569.998219
AACTCGA252.83388E-669.9981937
AGGGGGG65350.065.2852370
CTCGTAA453.8380676E-1062.2206239
AACTCGT650.059.22924837
TGCCGTA650.059.22924845
CGTATGA1550.058.70816841
GAAATCG308.359213E-658.3318336
CTCGAAA308.359213E-658.3318339
GCCGTAT601.8189894E-1258.3318346
TCGAAAG308.359213E-658.3318340
AGCCGTC800.056.87353545
ACGTCTT1600.056.87353547
GACCACG1850.056.755318
CGTCTAC1050.056.66520748
TGACGTC1550.056.4501645
CCGTATT750.055.99855847
Produced by FastQC (version 0.11.5)