FastQCFastQC Report
Wed 25 May 2016
SRR1781091_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781091_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213561
Sequences flagged as poor quality0
Sequence length76
%GC66

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7814736.592355345779424No Hit
CTTATACACATCTGACGCTGCCGACGACGCAAATAGTGTAGATCTCGGTG2140.10020556187693445No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGGCG150.002218439570.0316242
TACCGAA150.002222565569.9988323
CGGGTCT207.914523E-569.9988257
GTGCGCG207.914523E-569.9988254
CGCGTCT308.357676E-658.332367
GGCGCGT252.3868128E-455.999065
CTGCCGG452.6901034E-854.4435318
GTGTATT200.006925510752.5360221
CGAACGA200.00694478752.4991226
GGTGTCG200.00694478752.4991256
GCGCGTC407.456292E-752.499126
CCAATGT200.00694478752.4991244
TCGGGCG200.00694478752.4991211
CGACCGG200.00694478752.4991270
TTCGTCC200.00694478752.4991220
TGCGCGG200.00694478752.499125
CGCTACA200.00694478752.4991263
GCTTAGA200.00694478752.4991264
GTCCGGG200.00694478752.4991234
CGGCTCG200.00694478752.4991248
Produced by FastQC (version 0.11.5)