FastQCFastQC Report
Wed 25 May 2016
SRR1781091_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781091_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213561
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT4868922.798638328159168No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG63052.9523180730564103No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC22201.0395156419009088No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTAGTATGCCGT18090.8470647730624973No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC11310.5295910770224901No Hit
ATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTCTT9710.45467103075936155TruSeq Adapter, Index 2 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTC8610.4031634989534606No Hit
CTTATACACATCTCCGAGCCCACGAGACAATCCTGTATCTCGTATGCCGT6940.3249657006663202No Hit
ATTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT6690.31325944343770634No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGACGT5110.23927589775286687No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTAAGCCGT4110.1924508688384115No Hit
CTTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG3980.1863636150795323No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTAGTATGCCG3750.17559385842920758No Hit
CTTTACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTC3580.16763360351375017No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3370.15780034744171453No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC3060.1432845884782334No Hit
TTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTC2930.1371973347193542No Hit
CTTAATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG2520.1179990728644275No Hit
CTTATACACATCTCCGAGCCAACGAGACCATCCTGTATCTCGTATGCCGT2360.11050706823811465No Hit
CCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG2310.10816581679239187No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTACG150.00222259569.998656
GCGCCGG150.00222259569.998611
TTCCGTA207.9146535E-569.998645
GCCGACA301.0204349E-769.998646
GCCGAAA150.00222259569.998646
CAGTGCG150.00222259569.998632
TCGTAAT150.00222259569.998640
ACGTAAG150.00222259569.998640
AGGGGGG59100.065.083370
CGTCTAC401.0617441E-861.2487748
ATCGTAT352.9648436E-759.998839
TCGTATT650.059.22958440
TCTACAG308.357842E-658.3321650
TACGAGA308.357842E-658.3321613
TGCCGAC601.8189894E-1258.3321645
GACGTAT553.6379788E-1157.2715846
AGCCGTC1350.057.0358945
TGCCGTA3650.056.57420345
GCCGTAT3100.056.4504846
TGCTTTA509.767973E-1055.99887555
Produced by FastQC (version 0.11.5)