FastQCFastQC Report
Wed 25 May 2016
SRR1781090_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781090_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162833
Sequences flagged as poor quality0
Sequence length76
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6406339.34276221650402No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGTCG150.002221505269.99969552
GATTGCG150.002221505269.99969517
CGAAGGA150.002221505269.99969535
GCCGTAT308.348177E-658.33307654
TGGTCGC452.6844646E-854.44420249
GTCGCCG407.444596E-752.49976751
GGTCGCG200.006941498752.49976750
CTAAGCT200.006941498752.49976763
CCTCGGG200.006941498752.49976718
CCGTCTG200.006941498752.49976711
CGTAGGG200.006941498752.49976715
GCCTCGG200.006941498752.49976717
GAGCCGC200.006941498752.49976738
ATTGCGC200.006941498752.49976718
TCGCCGT352.0795671E-549.99978352
CGTATCA352.0795671E-549.99978356
CGCCGTA352.0795671E-549.99978353
GTGGTCG506.157825E-848.99978348
CGGTGGT604.8967195E-946.66646246
CCGTATC404.5774832E-543.74980555
Produced by FastQC (version 0.11.5)