FastQCFastQC Report
Wed 25 May 2016
SRR1781090_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781090_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162833
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT4031824.76033727807017No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG59553.657121099531422No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC21701.3326537004169916No Hit
ATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCTT12960.7959074634748484No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC12640.776255427339667No Hit
ATTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT12040.7394078595862018RNA PCR Primer, Index 19 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTC7780.47779012853659886RNA PCR Primer, Index 19 (95% over 22bp)
CTTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG4020.2468787039482169No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGATAAAATCTCGTATGCCGT3990.2450363255605436No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAATCTCGTATGCCGTC3960.24319394717287035No Hit
CTTTACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTC3940.24196569491442152No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTC3110.19099322618879463RNA PCR Primer, Index 19 (95% over 22bp)
CTTATACACATCTCCGAGCCAACGAGACGCGTTAAAATCTCGTATGCCGT3080.1891508478011214RNA PCR Primer, Index 19 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCT2920.17932482973353067No Hit
CATATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT2690.16519992876136902RNA PCR Primer, Index 19 (95% over 21bp)
TCTTAATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC2650.16274342424447133No Hit
CTTATACACATCTCCGAGACCACGAGACGCGTTAAAATCTCGTATGCCGT2630.16151517198602247RNA PCR Primer, Index 19 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCAGT2610.16028691972757367No Hit
CTTAATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG2600.15967279359834924No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2470.15168915391843177No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAAAAATCTCGTATGCCGT2310.14186313585084104No Hit
CCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG2060.12650998262023053No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGACGT2030.1246676042325573No Hit
CTTATACACATCTCAGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT1910.11729809068186424RNA PCR Primer, Index 19 (95% over 21bp)
CTTATACACATCTCCGAGCACACGAGACGCGTTAAAATCTCGTATGCCGT1640.1007166851928049RNA PCR Primer, Index 19 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAC150.002222200969.9941659
GCGTTTA150.002222200969.9941629
TGACGAC207.911492E-569.9941645
TACGAGA150.002222200969.9941613
CGACTAA207.911492E-569.9941648
GACGACT252.831448E-669.9941646
AGGGGGG51750.064.3811170
TCCGAGA1450.060.33979413
GACGTAT352.961715E-759.99546
TGACGTA352.961715E-759.99545
TAAGCCG352.961715E-759.99543
ACGCGAT2000.059.49503727
CGCGATA2000.059.49503728
CCGAGAC1250.058.79509714
AGCGTTA900.058.3284728
AAGCCGT308.352086E-658.3284744
CTGATAG308.352086E-658.3284754
GCGATAA2200.057.26795229
GAGACGA800.056.8702624
CGTCTAC750.055.99533548
Produced by FastQC (version 0.11.5)