FastQCFastQC Report
Wed 25 May 2016
SRR1781089_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781089_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227203
Sequences flagged as poor quality0
Sequence length76
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8837038.89473290405496No Hit
CTTATACACATCTGACGCTGCCGACGACGCAAATAGTGTAGATCTCGGTG2770.12191740425962685No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC207.9152E-569.9995656
TGCGCGG207.9152E-569.9995655
TACACGT207.9152E-569.9995655
TCGGATT150.00222265769.9995630
AACGCAC150.00222265769.9995649
ATGCGCG150.00222265769.999564
CGCGTCT352.9654075E-759.9996227
ATACACG308.358873E-658.3329624
CGACGAG553.6379788E-1157.27236622
GGTCTCG553.6379788E-1157.27236641
CGTATCA750.055.9996556
GCGCGTG252.3870167E-455.9996456
GCTGCGG252.3870167E-455.99964517
TGCGGGT252.3870167E-455.9996455
CGCAAGG252.3870167E-455.99964512
GTGCGCG509.767973E-1055.9996454
CCGCAAG252.3870167E-455.99964511
GTAGATC700.054.99965738
GCCGTAT653.6379788E-1253.8458154
CGGTGGT1050.053.33299646
Produced by FastQC (version 0.11.5)