FastQCFastQC Report
Wed 25 May 2016
SRR1781089_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781089_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227203
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT5781325.44552668758775No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG80473.5417666139971744No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC26681.1742802691865866No Hit
ATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTT17870.7865213047362931No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC15530.6835297069140812No Hit
ATTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT12870.5664537880221653No Hit
CTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC12430.5470878465513219No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAATAAATCTCGTATGCCGT9980.4392547633613993No Hit
CTTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG5660.2491164289203928No Hit
CTTTACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC5300.23327156771697555No Hit
TTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC4930.2169865714801301No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4440.19541995484214555No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC4220.18573698410672396No Hit
CATATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT4150.18265603887272616No Hit
CTTATACACATCTCCGAGCCAACGAGACCCAAATAAATCTCGTATGCCGT4060.17869482357187183No Hit
TATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCT3790.16681117766930895No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATAATCTCGTATGCCGTC3650.16064928720131336No Hit
CTTAATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG3600.1584486120341721No Hit
CTTATACACATCTCCGAGACCACGAGACCCAAATAAATCTCGTATGCCGT2900.12763915969419418No Hit
CCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG2680.11795618895877255No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCAGT2670.11751605392534428No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAAAAAATCTCGTATGCCGT2670.11751605392534428No Hit
CTTATACACATCTCAGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT2530.1113541634573487No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAAATAAATCTCGTATGCCGT2330.1025514627887836No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATATCGTATGCCGT2320.10211132775535534No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTC150.002222600970.0000120
CACGGTT207.9149526E-570.0000149
TGGACGG150.002222600970.0000123
GACTGTG150.002222600970.0000140
GACTCGC150.002222600970.0000144
ACGGTTA207.9149526E-570.0000150
AGGGGGG73800.065.4471570
CGTCTAC700.065.0000148
TCTTTAT2200.063.636361
CGTCTTA800.061.25000448
CACGTAT401.0621079E-861.25000439
CCTTATA352.9652801E-760.0000041
CCAAGAG800.056.87500420
GCACGGT252.3869416E-456.00000448
ACAAGTG252.3869416E-456.00000427
TGCACGG252.3869416E-456.00000447
TTCCTTA252.3869416E-456.00000436
CACTTGT252.3869416E-456.00000430
ATACAGG252.3869416E-456.0000046
TATGCCG96100.053.4287243
Produced by FastQC (version 0.11.5)