FastQCFastQC Report
Wed 25 May 2016
SRR1781088_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781088_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119731
Sequences flagged as poor quality0
Sequence length76
%GC85

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8127867.88383960711928No Hit
CTTATACACATCTGACGCTGCCGACGACGCAAATAGTGTAGATCTCGGTG1890.1578538557265871No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGG207.8999146E-569.9995942
AGATCTC207.8999146E-569.9995940
GCCATAG150.002220077469.9995925
TGCATAG150.002220077469.9995949
GCCGTAT207.8999146E-569.9995954
CGGTGGT352.9534567E-759.9996446
TCGCCGT308.334804E-658.3329952
GTCGCCG308.334804E-658.3329951
TGCGGGC308.334804E-658.3329919
CTGCCGG308.334804E-658.3329918
GTGCGCG308.334804E-658.332994
GCGAGGG308.334804E-658.3329924
CGGGTGT252.3824339E-455.9996647
CGGCGAG252.3824339E-455.99966422
CGTATCA252.3824339E-455.99966456
TGCGCGT252.3824339E-455.9996645
GATCTCG252.3824339E-455.99966441
CGCCGTA252.3824339E-455.99966453
CTTGTGC200.006925612752.5216261
GTATTAC200.006925612752.5216261
Produced by FastQC (version 0.11.5)